Coding

Part:BBa_K5257022:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


DogTag-MerA_2D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 341
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 341
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 341
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 341
    Illegal NgoMIV site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 276


Design Notes

Codon optimization of the linker for its synthesis was performed by replacing the sequence of ggaggaggaggtagt (BBa_K2549053) with ggaggaggtggatca (GGGGS)



Source

Only iGEM parts, Each iGEM part was previously characterized by bibliography.

Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem, 21(15), 2126-2131. https://doi.org/10.1002/cbic.202000102


References